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Tryptone 35 Yeast extract 20 (NH4)2SO4 3
Cold Spring harbour Laboratory
0002% Sulfate (SO4) <0
The antibiotic is an irreversible inhibitor of protein synthesis
2 at 25°C Suspend 26 gram powdered medium in 1 litre distilled water
FM Minimal medium w/o Glutamic acid and w/o Lysine Size:250g Tryptone 35 Yeast extract 20This media is based on well defined formulations of mineral salts, vitamins and amino acids. Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH. FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5. SIH medium is a newly developed modification of FM with regards to
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