Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Inositol Size:500g 4°C Water KF 0

4°C Water KF 0

95g in 1L of Distilled or De-ionised Water

Soya Peptone 10 Yeast extract 5 NaCl 10 Agar 15 Final pH 7

2 at 25°C Suspend 20 gram powdered medium in 1 litre distilled water

Yeast extract 5 Dextrose 30 Total 35

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Inositol Size:500g 4°C Water KF 0Inositol is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of inositol or to replace this vitamin. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room temperature